柚皮素调控miRNA-21对缺氧再复氧后心脏微血管内皮细胞的影响

摘要/Abstract

摘要： 目的研究柚皮素调控miR-21来干预心肌梗死介导的心脏微血管内皮细胞凋亡的作用和机制。方法培养原代人心脏微血管内皮细胞,建立体外缺血再灌注的细胞模型,将细胞分为对照组、缺氧再复氧组（I/R）、nar组（柚皮素50 μg/mL）、I/R+miR-21 mimic组（细胞转染miR-21模拟物后在缺氧再复氧条件下）、I/R+nar+miR-21 mimic组（细胞转染miR-21模拟物后在缺氧再复氧条件下加上柚皮素）、I/R+miR-21inhibtor组（细胞转染miR-21抑制物后在缺氧再复氧条件下）。采用PCR检测miR-21的表达,CCK-8法检测细胞存活率。结果在I/R条件下观察柚皮素对HCMEC细胞的影响,nar+I/R组比I/R组的细胞凋亡率显著减少[（17.08±2.06）% vs （33.51±1.66）%],差异具有统计学意义（P<0.01）。与 I/R 组相比,I/R+nar组的miR-21表达增加,差异具有统计学意义（0.24±0.046 vs 2.23±0.38,P<0.01）。I/R+miR-2 mic组与 I/R组（25.15±1.57 vs 33.51±1.66）相比,前者表现出较少的细胞凋亡,差异具有统计学意义（P<0.01）。与I/R组（33.51±1.66）相比,I/R+miR-21inhib（43.77±4.71）显著促进了细胞凋亡,差异有统计学意义（P<0.05）。结论柚皮素通过调控miR-21介导了人心脏微血管内皮细胞的抗凋亡作用。

关键词: 心肌梗死, 心脏微血管内皮细胞, 柚皮素, miR-21

陈柳. 柚皮素调控miRNA-21对缺氧再复氧后心脏微血管内皮细胞的影响[J]. 心血管病防治知识, 2026, 16(3): 41-44.

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